Montpellier Scientific Community



Functional Proteomics Laboratory

Director: Michel Rossignol
Research area
The LPF (Functional Proteomics Laboratory) is an INRA Research Unit specialised in proteomics, a recent molecular science that studies the sets of proteins active in cells, downstream to genome expression. Beyond cataloguing proteins in a given physiological context, the focus is given on the reversible modifications of proteins that determine their function. The LPF main concern is the posttranslational signalling of environmental fluctuations in plants, with the goal of an integrated knowledge about the networks of posttranslational events that lead to the final molecular responses to abiotic stresses. These quantitative approaches take advantage of the IBiSA mass spectrometry proteomics platform hosted by the lab and are associated to molecular physiology studies through collaborations.
Research highlights
  • Methylation of aquaporins in plant plasma membrane
  • Proteomic analysis of phosphorylated proteins
  • Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify phosphorylation sites in secondary active transporters
Staff profile
 
Total permanent staff Total Scientists Scientists with "HDR" [1] Post-doc fellows PhD
12
7
1
0
2
[1]French university degree for confirmed thesis supervisor
    
         
Research teams
  • Protein nitrosylation
  • Protein phosphorylation
  • Protein tagging by Ub and SUMO polypeptides
  • Proteome analysis platform
Platforms and other tools
Proteome analysis platform: robotics, mass spectrometry, bioinformatics
Most important international partnerships
  • EU program GVE, ended 2005 (B, D, GB)
  • ERA-PG program ProteinStorage (D, SP)
  • Bilateral collaborations (CZ, I, IRL, P, PL)
Facts and figures
Publications in international ranking journals
2007: 8
2006: 7
2005: 6
Representative publications
Chevalier F, Martin O, Rofidal V, Devauchelle AD, Barteau S, Sommerer N, Rossignol M (2004) Proteomic investigation of natural variation between Arabidopsis ecotypes. Proteomics, 4, 1372-1381.

Nacry P, Canivenc G, Muller B, Azmi A, Van Onckelen H, Rossignol M, Doumas P (2005) A Role for Auxin Redistribution in the Responses of the Root System Architecture to Phosphate Starvation in Arabidopsis. Plant Physiol., 138, 2061-2074.

Chevalier F, Centeno D, Rofidal V, Tauzin M, Martin O, Sommerer N, Rossignol M (2006) Different Impact of Staining Procedures Using Visible Stains and Fluorescent Dyes for Large-Scale Investigation of Proteomes by MALDI-TOF Mass Spectrometry. J. Proteome Res., 5, 512-520.

Faurobert M, Mihr C, Bertin N, Pawlowski T, Negroni L, Sommerer N, Causse M (2007) Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening. Plant Physiol., 143, 1327-1346.

Segonzac C, Boyer JC, Ipotesi E, Szponarski W, Tillard P, Touraine B, Sommerer N, Rossignol M, Gibrat R (2007) Nitrate Efflux at the Root Plasma Membrane: Identification of an Arabidopsis Excretion Transporter Plant Cell, 19, 3760-3777.