Elucidation and manipulation of plant genomes is a key point for basic biology research and crop improvement. Developing genome edition biotechnological tool in coffee would greatly accelerate research and breeding of news varieties more adapted to current climate change.
The main objective is to reconstruct a functional CRISPR-Cas9 system in the two cultivated coffee. The main scientific breakthrough is to develop a new set of tools to achieve chirurgical gene knockout in coffee species and to asses its efficiency, hence leading to significant advances in functional genomics and renewing the range of possibilities in coffee breeding.
Bioinformatic tools and data integration into the coffee genome browser interface
Construction of 2 set of Binary vectors
Mutants regeneration and analysis
Development of algorithms to identify all sgRNA (single guide RNA) target sequences and all potential off-target for each sgRNA in coffee genome.
Identification of unique sgRNA target sequence in coffee genome coding sequence.
Data integration into the coffee genome browser interface (http://coffee- genome.org/).
Construction of 2 set of binary vector bearing one or two sgRNA, Cas9 genes and GFP.
C. canephora agrobacterium mediated genetic transformation protocol.
Regeneration and analysis of a large population of plants. Demonstration of CRISPR/Cas9 system efficiency in C. canephora.
The success of COFEDIT project will enable us, since 2017, to develop simplified and more efficient functional analysis tools as well as establishing in Agropolis a functional analysis platform available for the whole coffee community. These new tools will form the basis for developing new projects and new cooperation.
Project Number : 1403-015
Year : 2014
Type of funding : AAP OS
Project type : AAP
Research units in the network : AGAP DIADE
Start date :
01 Feb 2015
End date :
31 Jan 2016
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