To characterize the wheat responses to beneficial and harmful microbes by both transcriptomic (RNAseq) and proteomic (proteome and phosphoproteome) analyses to better understand the wheat-microbes interactions.
WP 1: Profiling of the wheat root-associated microbiomes
WP2 : Identification of inoculation parameters that maximize wheat performance under different environmental conditions
WP3 : Evaluation of molecular responses of wheat to beneficial microbes
WP4 : Field experiments in different agro-social-economic ecosystems
WP5 : Dissemination and training
1. Wheat inoculated with A. brasilense and G. mosseae (alone or combined) grew 2 to 3 times higher compared to control plants
(Fig 1 a&b).
2. The roots of plants inoculated by B. graminis weighed twiced over the control.
3. Glomus mosseae increase wheat growth and is able to reduce significantly the lesion length in leaves inoculated by the
pathogen Xanthomonas translucens (Fig 1 c).
The first results of the project show that wheat inoculation by arbuscular mycorrhizal fungi (G. mosseae) and symbiotic beneficial bacteria (A. brasilense and B. graminis) are compatible and produce significant phenotypes (growth, biocontrol). The next step is to analyze these plant phenotypes by transcriptomics and proteomics to decipher the mechanisms underlying the growth increase and resistance to the X. translucens infection.